The nucleotide sequence of 3-isopropylmalate dehydrogenase gene fromBacillus subtilis
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of the Bacillus subtilis phoR gene.
The nucleotide sequence of phoR, the positive and negative regulatory gene for alkaline phosphatase and phosphodiesterase formation in Bacillus subtilis, was determined. The sequence data predicted an open reading frame of 1,740 base pairs (579 amino acids) which overlaps the 5 base pairs of the preceding phoP coding sequence. The deduced amino acid sequence was significantly homologous with th...
متن کاملNucleotide sequence of the Bacillus subtilis developmental gene spoVE.
We have determined the nucleotide sequence of a 1159 bp DNA fragment containing the spoVE locus of Bacillus subtilis. The locus contained a single open reading frame of 293 codons. On the basis of the predicted amino acid sequence, the product of the spoVE gene is believed to be a protein with an Mr of 31,539. The amino-terminal portion of the spoVE gene was used to construct a translational fu...
متن کاملNucleotide sequence of the glyceraldehyde-3-phosphate dehydrogenase gene from Cryphonectria parasitica.
The gene for glyceraldehyde-3-phosphate dehydrogenase (gpd-l) of Cryphonectria parasitica, the chestnut blight fungus, was isolated by probing a genomic library with a PCR (1) amplified copy of the coding region of the Aspergillus nidulans homologue, gpdA (2). Presented below is the nucleotide sequence for the entire gpd-l coding region, 381 bp of the 5'-flanking region and 557 bp of the 3'-fla...
متن کاملNucleotide sequence of hamster glyceraldehyde-3-phosphate dehydrogenase mRNA.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used as an invariant control for gene expression analysis. Only three vertebrate GAPDH cDNAs have been hitherto isolated: human (1), rat (2) and chick (3). In order to get a probe for RNAse-mapping experiments on hamster {Cricetulus griseus) mRNAs, we constructed a cDNA library in pBluescript KSH (St...
متن کاملHigh-pressure-induced water penetration into 3-isopropylmalate dehydrogenase
Hydrostatic pressure induces structural changes in proteins, including denaturation, the mechanism of which has been attributed to water penetration into the protein interior. In this study, structures of 3-isopropylmalate dehydrogenase (IPMDH) from Shewanella oneidensis MR-1 were determined at about 2 Å resolution under pressures ranging from 0.1 to 650 MPa using a diamond anvil cell (DAC). Al...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.12.4988